Journal: Micromachines

Article Title: The Effect of Microfluidic Geometry on Myoblast Migration

doi: 10.3390/mi10020143

Figure Lengend Snippet: Migration behavior of primary myoblasts is dependent on the width of microchannels. ( a ) Representative fluorescent image of cells stained with Calcein AM in the proximal and distal chamber 48 h after seeding. The device consists of microchannel length 100 µm and variable widths (1.5–20 µm) with 6 repeating microchannels per width. ( b ) Normalized mean intensity of fluorescence in the distal chamber taken for each width from 1.5–20 µm sections. The mean intensity of fluorescence was normalized to the proximal chamber. ( c ) Quantification of the number of cells in the distal chamber after 48 h from 1.5–20 µm sections. To determine statistically significant differences in the cell count in the distal chamber as a function of microchannel width, a one-way ANOVA with a post hoc Tukey test was performed. Error bars represent standard error of the mean (SEM).

Article Snippet: During live imaging sessions of myoblasts stained with Calcein AM dye, the cells were maintained in a humidified chamber at 37 °C and 10% CO 2 (OKOLAB USA Inc., San Francisco, CA, USA).

Techniques: Migration, Staining, Fluorescence, Cell Counting